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The TopoJ plugins help you analyze surfaces of bright objects in image stacks. You can load an example via File › Open Samples › SinteredAlumina (2.6MB):
Compute Topography
To compute the Topography, call Analyze › TopoJ › Compute Topography:
The result looks like this:
Remove Slope
To remove the overall slope of the surface, click on the Analyze › TopoJ › Remove Slope menu item: