The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.
To install the plugin, activate the Angiogenesis update site and restart Fiji.
Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via Analyze › Sprout Morphology. The process consists of up to six dialogs:
Endothelial cell/Pericyte classification