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Name | Sprout Morphology |
Software | Fiji |
Author | |
Maintainer | |
Source | |
Status |
stable |
Category |
The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.
Installation
To install the plugin, activate the Angiogenesis update site and restart Fiji.
Usage
Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via Analyze › Sprout Morphology . The process consists of up to six dialogs:
General configuration

Bead detection

Sprout detection

Nucleus segmentation

Endothelial cell/Pericyte classification

Pericyte coverage

Publication
Eglinger J., Karsjens H., Lammert E., “Quantitative assessment of angiogenesis and pericyte coverage in human cell-derived vascular sprouts”, Inflammation and Regeneration 37(2): doi:10.1186/s41232-016-0033-2.