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Sprout Morphology

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The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.


To install the plugin, activate the Angiogenesis update site and restart Fiji.


Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via AnalyzeSprout Morphology. The process consists of up to six dialogs:

General configuration

Configuration dialog

Bead detection

Bead detection

Sprout detection

Sprout detection

Nucleus segmentation

Nucleus segmentation

Endothelial cell/Pericyte classification

Cell classification of ECs and pericytes

Pericyte coverage

Pericyte coverage measurement