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Sprout Morphology

Name

Sprout Morphology

Software

Fiji

Author

Jan Eglinger

Maintainer

Jan Eglinger

Source

on GitHub

Status

stable

Category

Analysis


The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.

Installation

To install the plugin, activate the Angiogenesis update site and restart Fiji.

Usage

Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via AnalyzeSprout Morphology . The process consists of up to six dialogs:

General configuration

Configuration dialog

Bead detection

Bead detection

Sprout detection

Sprout detection

Nucleus segmentation

Nucleus segmentation

Endothelial cell/Pericyte classification

Cell classification of ECs and pericytes

Pericyte coverage

Pericyte coverage measurement

Publication

Eglinger J., Karsjens H., Lammert E., “Quantitative assessment of angiogenesis and pericyte coverage in human cell-derived vascular sprouts”, Inflammation and Regeneration 37(2): doi:10.1186/s41232-016-0033-2.