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KymographBuilder › Yet Another Kymograph Fiji plugin

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KymographBuilder is Yet Another Kymograph Fiji plugin.

Features are :

  • Build kymograph from a straight or segmented line (a polyline).
  • Build kymograph for images with multiple channels.
  • Take into account the width of the line using maximum projection along its thickness.
  • Built from scratch and use the ImageJ2 Ecosystem.
  • Fast and easy to use.

How to use :

  • Draw a line: it can be a polyline and you can also set the thickness that will be taken into account by the plugin.
  • Launch KymographBuilder : PluginsKymographKymographBuilder
  • You’re done.


You can script the plugin. Here is an example:

  • Multi_Kymograph : The first kymograph bundled with Fiji, with support for multiple Line ROIs
  • Source code on GitHub :
  • Maven :;quick~kymographbuilder


KymographBuilder has been created by Hadrien Mary.

This work started in 2016 at the Gary Brouhard laboratory ( at McGill University.