Page history Edit this page How do I edit this website?
Original MediaWiki page
This page describes content relating to the Fiji Cookbook project. Click the logo for details.

Colour merge

The content of this page has not been vetted since shifting away from MediaWiki. If you’d like to help, check out the how to help guide!

Name

Colour merge

Software

ImageJ

Author

unknown

File

Colour_merge.class

Source

not found

Status

unknown

Category

Color Processing


Purpose

This plugin allows you to merge two grayscale stacks into a single RGB stack. It is useful e.g. to merge a brightfield with the corresponding fluorescence image, or two different dye images of the same series.

Documentation

Adapted from the MBF ImageJ manual:

The PluginsColor functionsColor Merge function gives the user the option of using the Difference arithmetic processing on the image stacks you select. This is not strictly a merge (when cyan and magenta merge they produce white, not yellow) but facilitates visualization of the separate channels1. You can perform a true merge if you turn off the “Difference” option.

Run the plugin and select the two images or stacks to be merged. It can be 8-bit, 16-bit, RGB… Select the desired colors from the drop-down options. uses the LUT that the image currently has (this is often the desired LUT). The "Difference" option performs a "difference" arithmetic operation rather than a "addition". If the "Pre-sub 2 from 1" option is checked the second image is subtracted from the first prior to merging.

References