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Save as animated gif

  1. Go to menu ImagesStacksToolsAnimation options…
  2. In the dialog, set the desired frames per second, or “Speed”.
  3. Select the stack to save, and go to menu FileSave AsAnimated Gif …


PS. in order to include overlays in the animated gif, such as ROI or scale bar overlays, these must first be converted to pixel data by flattening the image.

  1. Go to menu ImageOverlayFlatten

or use ⌃ Ctrl / ⌘ ⌃ Ctrl on a PC ⌘ command on a Mac  + ⇧ Shift + F.