[ImageJ-bugs] [Bug 1025] New: Stitching in Fiji is not using stage coordinates from CZI correctly

bugzilla at fiji.sc bugzilla at fiji.sc
Fri Mar 13 02:54:36 CDT 2015


http://fiji.sc/bugzilla/show_bug.cgi?id=1025

            Bug ID: 1025
           Summary: Stitching in Fiji is not using stage coordinates from
                    CZI correctly
           Product: Fiji
           Version: unspecified
          Hardware: PC
                OS: Windows
            Status: NEW
          Severity: major
          Priority: P5
         Component: Plugins
          Assignee: imagej-bugs at imagej.net
          Reporter: sebrhode at googlemail.com

Created attachment 240
  --> http://fiji.sc/bugzilla/attachment.cgi?id=240&action=edit
Comparison for ZEN and Fiji Stitching results

Hi,

I just tried to compare the ZEN stitching with the Fiji one, since Fiji
Stitching has some very nicefeatures, we are still missing ...

The test data set can be found here:

https://dl.dropboxusercontent.com/u/623476/Overview_10X_DAPI.czi

The problem is, that the Fiji stitching is "moving" some tiles to a position,
where they do not belong. And the result has empty tiles, while the original
CZI images does not have any empty tiles.

The original area is a rectangle, so the result must also be a rectangular tile
area (since this is knwon from the stage coordinates, since I use "Positions
from File" & "Defined by image Metadata"). But in Fiji the result looks
different --> see attachment.

It seems to me, that Fiji is moving some tiles, where is "not much to stitch"
to some XY position instead of leaving them at their original positions, when
the tile is rather "empty" (just an idea).

Do you have any idea, how solve this issue?

Sebi

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