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File:SPIMScheme.png
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Schematic drawing of a Selective Plane Illumination Microscope
The specimen embedded in agarose is illuminated by a laser light sheet, the CCD camera behind the objective is focussed on the center of the light sheet. Optical sectioning is achieved by moving the sample through the light sheet. Multiple acquisitions of the sample can be taken by rotating the agarose column. The water in the sample chamber can be cooled by a Peltier device to slow down developmental processes.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 11:15, 27 May 2010 | | 775 × 775 (180 KB) | Axtimwalde (talk | contribs) | Schematic drawing of a Selective Plane Illumination Microscope The specimen embedded in agarose is illuminated by a laser light sheet, the CCD camera behind the objective is focussed on the center of the light sheet. Optical sectioning is achieved by mov |
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