The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.
To install the plugin, activate the Angiogenesis update site and restart Fiji.
Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via Analyze › Sprout Morphology . The process consists of up to six dialogs:
Endothelial cell/Pericyte classification
Eglinger J., Karsjens H., Lammert E., “Quantitative assessment of angiogenesis and pericyte coverage in human cell-derived vascular sprouts”, Inflammation and Regeneration 37(2): doi:10.1186/s41232-016-0033-2.