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Sprout Morphology

    Name

    Sprout Morphology

    Software

    Fiji

    Author

    Jan Eglinger

    Maintainer

    Jan Eglinger

    Source

    on GitHub

    Status

    stable

    Category

    Analysis

    The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.

    Installation

    To install the plugin, activate the Angiogenesis update site and restart Fiji.

    Usage

    Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via Analyze › Sprout Morphology . The process consists of up to six dialogs:

    General configuration

    Configuration dialog

    Bead detection

    Bead detection

    Sprout detection

    Sprout detection

    Nucleus segmentation

    Nucleus segmentation

    Endothelial cell/Pericyte classification

    Cell classification of ECs and pericytes

    Pericyte coverage

    Pericyte coverage measurement

    Publication

    • TODO citation: Eglinger, J.; , ; Karsjens, H. (2017), Quantitative assessment of angiogenesis and pericyte coverage in human cell-derived vascular sprouts, Inflammation and Regeneration, ISSN