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    GDSC SMLM plugins provide various tools for single molecule localisation analysis.

    • Fit an image, or series of images, using a 2D Guassian Point Spread Function (PSF)
    • Save results to a table, a file, an image and/or to memory
    • Trace or cluster localisations through time to identify molecules
    • Correct drift in long time course images
    • Estimate fluorophore dark-time and blinking rate
    • Create localisation density images
    • Create custom PSFs from calibration images
    • Create simulated data using a Gaussian or custom PSF with configurable molecule populations and diffusion
    • Calibrate the gain and read noise of your microscope camera
    • Estimate noise in an image
    • Estimate resolution using Fourier Ring Correlation (FRC)

    See the latest documentation on ReadTheDocs.

    Find the code on GitHub.