Edit page History

Colocalization Threshold

    Name

    Colocalization Threshold

    Software

    ImageJ - Fiji

    Author

    Tony Collins (and others?)

    Maintainer

    Daniel James White

    File Colocalization.jar
    Source

    on GitHub , modified from MBF ImageJ

    Status

    no longer actively supported, unlikely to be stable enough for real use. Use Coloc_2

    Purpose

    Sets the thresholds for colocalization analysis, and also calculates Manders coefficients, 2D histogram / scatter plot, and other stats.

    Documentation

    Note: this plugin is now deprecated. You should use the newer Coloc 2 plugin instead.

    See the great documentation for this plugin at Colocalization Analysis#Colocalization_Threshold. The method is that implemented in Costes et al. This uses a Pearson’s correlation above and below the thresholds to iteratively find where the correlation between the two images is 0 and sets the thresholds there. It is pretty robust and importantly is reproducible and non subjective.

    Use of this plugin should be followed by use of the Colocalization Test plugin, to see of the results are statistically significant and better then random overlap of the signal in the images.

    See also the Colocalization Analysis tutorial.