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2017-12-04 - Fiji + KNIP hackathon

    From Monday, December 4, 2017 through Friday, December 15, 2017, the Max Planck Institute of Molecular Cell Biology and Genetics hosts ~50 developers at the Center for Systems Biology in Dresden, Germany for a hackathon to develop ImageJ2 and Fiji core infrastructure and plugins.

    https://gitter.im/fiji/hackathon_dd_2017

    Voluntary hackathon calendar

    https://tinyurl.com/ybjcq9qw

    Hackathon google doc

    https://docs.google.com/document/d/1h4uCt4PAEdeGQQwwVZC73o_Anq6-AI2KZiSzxY8kWng/edit

    Hackathon on Twitter (#hackdd17)

    https://twitter.com/hashtag/hackdd17?vertical=default&src=hash

    Technical Discussions

    N5, not HDF5

    For more info on N5, check out the github repository here.

    • feels like HDF5, but stores chunks(blocks) in separate files in the file system.
    • is a Java library, but Constantin Pape already wrote a C++/python version of it: z5 (also matches “zarr” library), https://github.com/constantinpape/z5
    • attributes are stored in an additional JSON file
    • Discussion: should we define standard now as to how data should be stored in there to prevent an emergence of a zoo of different flavors as there is for HDF5?
      • how to do time series where each timestep / angle could have different image size
      • if we want a general “N5 viewer” for images, we’d have to add calibration data
      • put this information around the N5 dataset, because it behaves more like a dataset within an H5 file.
      • Perhaps make it versioned? Because a duck is not always a duck…
    • why another file format?
      • parallel writes (awesome for clusters with shared filesystem)
      • there is a special type for label blocks
      • blocks can have a halo
      • the block grid does not need to be filled dense, some blocks could be missing
      • couldn’t this just be another flavor of HDF5?
    • are parallel writes to the same block prevented by some kind of locks?
    • the HDF5 team should be included in the discussions to learn from their mistakes - there is lots of information on parallel writing of HDF5 files out there
    • Try to write a N5 dataset into a FUSE filesystem/file??? Could this be a work-around for the many-small-files issue?

    BigDataViewer

    • There is a fork of BigDataViewer for JavaFx. 1
    • BigDataViewer will be splitted into a UI independent part, and the Swing UI. This will make it possible to merge the JavaFx and Swing Version of BigDataViewer .

    We discussed opportunities to improvement for the Bdv design:

    • Slim the core of bdv, make it less smart and more predictable.
    • Bdv handle for different UI toolkits,
    • Use property pattern for settings like active source …
    • Actively add/remove views
    • Update views (force cache-dropping) - for BDV add/remove views
    • Access to color settings, overlap rendering, …
    • Allow Grouping of views (externally is ok)
    • Consider Accumulator / Composite that works with type other than ARGBType
    • Make it easy to disable or replace Dialogs
    • Make it easy to remove an overlay
    • BdvVistools show function for converters/volatile

    Matrix and Vector libraries

    • Discussion notes: https://docs.google.com/document/d/12a_9AhFMJywm7y-SfZHuWB5cXwEkpCGt03Sxq8JVfMI/edit
    • Compared different matrix and vector libaries: https://github.com/imglib/imglib2-matrix-shootout
    • Conclusion:
      • use ojAlgo for N-D matrix operations
      • vector3 and vector4 is a complicated conclusion. probably use mastodon and JOML

    ImageJ/Fiji parallelization

    • initial focus on embarassingly parallel data
    • specific parallel (cluster) implementation will be abstracted
    • from the system architecture point of view, the design can be inspired by the search bar feature implementation
    • started use case definition

    Hackathon Progress

    ilastik - (Dominik Kutra, Carsten Haubold)

    • developed an ImageJ2 plugin that allows to stream raw data and predictions from the work in progress ilastik processing backend: https://github.com/ilastik/org.ilastik.bdvsource
    • discussed with Philipp Hanslovsky about the benefits of using the N5 format for communication
      • the label block format together with the fact that datasets can be stored sparse could be a great foundation for label storage / communication
      • played around with Constantin Pape’s C++/Python N5 reader as available format on the ilastik server
      • noticed that the only thing needed to serve a N5 dataset on the web is to serve the directory contents. Philipp wrote a N5 REST reader for BigCAT to talk to the ilastik server that way
    • worked on some caching problems in the ilastik server
    • discussed possible interfaces between Matthias Arzt’s LabKit and ilastik-backend for interactive segmentation in ImageJ/BigDataViewer with an ilastik classifier backend

    SciView - (Kyle Harrington, Ulrik Günther, Curtis Rueden)

    • set up automatic deployment of Fiji update site that works on all operating sustems
    • imglib2 images as textures on 3D objects
    • imagej2-style logging
    • public availability of SciView plugin

    ImageJ server - (Curtis Rueden, Petr Bainar)

    • discussed its design with respect to the ImageJ/Fiji architecture as well as its future use cases
    • identified the most imminent issues, raised them on GitHub and brainstormed possible solutions
    • worked on a new JavaScript client with enhanced UI, making use of the Angular framework
    • demoed the new client to the community
    • encouraged members of the community to use annotations specifying whether a SciJava plugin can run headless or not

    SciJava - (Curtis Rueden)

    ImageJ Ops - (Curtis Rueden)

    ImageJ Legacy - (Curtis Rueden)

    ImageJ Launcher - (Curtis Rueden)

    Bio-Formats - (Curtis Rueden)

    • In response to a question from Christian Tischer and hoerldavid , filed a PR to enable the high-level Bio-Formats API to accept file patterns directly (openmicroscopy/bioformats#3019 ).
    • Fixed new and existing Java-8 installations of Fiji to work properly with Bio-Formats again (1). This entailed creating a script that instructs users of the old Java 6 version of Fiji to please enable the Fiji-Legacy update site, and instructs users of the new Java 8 version of Fiji to please enable the Java-8 update site.

    ClearControl / ClearVolume / ClearCL (Robert Haase, Loic Royer)

    • Made Image Quality / Focus measurements from Royer et Al. Nat. Biotechnol. (2016) available as Fiji plugin on a preliminarily update site
    • Added support for image quality measurements in regions, tissue depth (rings) or tiled over the whole image
    • Fixed bugs regarding discovery of OpenCL files distributed using ClearCLs mechanism
    • Adapted ClearControl software to run on another microscope
    • Revived code for controlling filter wheels and deformable mirrors
    • Merged changes from Max to ClearControl-lightsheet preparing his time stepping procedures

    ImgLib2 IJ - (Matthias Arzt)

    Labkit - (Matthias Arzt)

    • Improved Usability of the Labkit plugin. (maarzt/imglib2-labkit )
    • Benchmark of different sparse ROI implementations.

    ImgLib2 ROIs - (Alison Walter, Tobias Pietzsch, Curtis Rueden)

    • Changes related to: imglib2/imglib2-roi#29

      • Improved transform operation for ROIs
      • Fixed boundary computation for transformed ROIs
      • Added knownConstant to ROIs for determining if a ROI will always return false or true
      • Removed type parameter for wrappers in Masks static methods, BoolType by default

    ImageJ-OMERO - (Alison Walter, Josh Moore)

    BigCAT (Philipp Hanslovsky)

    • relevant bigcat branch
    • Added Painting Functionality
      • Multi-scale WIP
      • Used for dense annotations but can be used for label creation for classifier training
    • Added open dialogs for N5 and HDF5
      • Auto-discovery
      • Channels
      • Time series
      • multi-scale
      • User guidance in case of invalid input
    • Created a mock server to access local file system through http rest api
    • Confirmed time series and multi-channel capabilities
    • Connected to ilastik backend (Dominik Kutra, Carsten Haubold)
    • Tried to re-introduce scenery for 3D rendering together with Ulrik Günther (WIP)

    scyjava & imglyb (Philipp Hanslovsky, Curtis Rueden)

    • Made plan to separate Java and Python code in imglyb
    • Created scyjava python wrapper around pyjnius to allow for runtime resolution of jars/classes that are not on the classpath yet.
      • For now: Facilitiate groovy grape, might switch to more generic scijava-grab library later on.
      • Removes fat jar requirement of imglyb and will make it easier to distribute
      • Currently WIP as jnius.autoclass does not accept custom class loaders as parameter: (kivy/pyjnius#70 )

    Image Sequence Registration Plugin (Christian Tischer)

    • Wrote an ImageJ2 plugin for image sequence registration
    • Source code on github
    • Installation via new Fiji Update site EMBL-CBA
    • The code is written such that additional registration modalities can be readily added
    • Current functionality
      • Accepts N-dimensional input, allowing the user to select which axes to register
      • Translational registration using phase-correlation
      • Translational and rotational registration, using phase-correlation for translation and “brute-force” testing of all rotations within user specified range
      • Region of interest selection

    Mastodon (Jean-Yves Tinevez, Tobias Pietzsch)

    • User-assigned tags
    • Editable TrackScheme graph
    • Revised action handling to allow to switch keymaps
    • Navigate-to-branch-child/parent/sibling actions
    • Preferences dialog
      • Render settings for BDV view
      • Keymap configuration
    • Code cleanup

    TrackMate & MaMuT (Ulrik Günther, Kyle Harrington, Jean-Yves Tinevez)